4B48
Bacterial translation initiation factor IF2 (1-363), complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 295 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.020, 61.950, 160.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.345 - 2.800 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b3x |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1278)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.120 | 0.880 |
Number of reflections | 11636 | |
<I/σ(I)> | 15.4 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |