4B45
CetZ2 from Haloferax volcanii - GTPgS bound protofilament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.427, 43.224, 48.450 |
| Unit cell angles | 68.87, 75.34, 86.90 |
Refinement procedure
| Resolution | 40.284 - 2.100 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CETZ4 MODEL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.280 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.940 |
| Number of reflections | 20523 | |
| <I/σ(I)> | 8.1 | 1.5 |
| Completeness [%] | 97.9 | 96.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 3.15 M AMMONIUM SULFATE, 0.1 M NA CITRATE PH 5 |
