4B43
Bacterial translation initiation factor IF2 (1-363), apo form, double mutant K86L H130A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 295 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.787, 61.971, 162.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.283 - 1.937 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.23550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b3x |
RMSD bond length | 0.015 |
RMSD bond angle | 1.367 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1278)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.080 | 0.890 |
Number of reflections | 34540 | |
<I/σ(I)> | 8.9 | 1.4 |
Completeness [%] | 96.5 | 81.7 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6 |