4B3W
Crystal structure of human cytoglobin H(E7)Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.844, 70.054, 102.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 2.800 |
| R-factor | 0.21371 |
| Rwork | 0.207 |
| R-free | 0.27727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ut0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 9092 | |
| <I/σ(I)> | 10.9 | 3.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 18% PEG 4000, 0.2M NACL, 0.05M NA-ACETATE (PH 4.1-4.6), 0.01M K-FERRICYANIDE, 1MM KCN |
