4B3N
Crystal structure of rhesus TRIM5alpha PRY/SPRY domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-30 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.837, 98.733, 110.332 |
Unit cell angles | 90.00, 122.99, 90.00 |
Refinement procedure
Resolution | 49.420 - 3.300 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.24843 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1ANF 2VOL |
RMSD bond length | 0.010 |
RMSD bond angle | 1.416 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.170 | 0.890 |
Number of reflections | 22400 | |
<I/σ(I)> | 6.69 | 1.39 |
Completeness [%] | 96.5 | 97.1 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 6% (W/V) GLUCOSE, 6% (W/V) TREHALOSE, 100 MM MES PH 6.2 AND 25% PEG 3350 |