4B3H
Crystal structure of Mycobacterium tuberculosis fatty acid beta- oxidation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 249.460, 134.250, 118.400 |
| Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.300 |
| R-factor | 0.19687 |
| Rwork | 0.195 |
| R-free | 0.22417 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wdk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.985 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 153278 | |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2 M AMMONIUM SULPHATE, 0.1 M TRIS PH 8.5 |
