4B3F

crystal structure of Ighmbp2 helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	116.566, 76.717, 88.536
Unit cell angles	90.00, 107.33, 90.00

Refinement procedure

Resolution	55.639 - 2.500
R-factor	0.1934
Rwork	0.190
R-free	0.25230
Structure solution method	MIRAS
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.181
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.040	0.510
Number of reflections	25840
<I/σ(I)>	16	2.3
Completeness [%]	91.8	99.7
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1
1