4B3D
Humanised monomeric RadA in complex with 5-methyl indole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.280, 87.246, 62.628 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 40.266 - 1.589 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PZN CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.620 | 1.690 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.610 |
| Number of reflections | 57763 | |
| <I/σ(I)> | 13.76 | 2.19 |
| Completeness [%] | 99.2 | 96.5 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
