4B3B

Humanised monomeric RadA in complex with FHTA tetrapeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-14
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	40.227, 60.589, 87.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.286 - 1.193
R-factor	0.1414
Rwork	0.140
R-free	0.16710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PZN CHAIN A
RMSD bond length	0.010
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.290	1.260
High resolution limit [Å]	1.190	1.190
Rmerge	0.060	0.240
Number of reflections	64405
<I/σ(I)>	16.06	5.14
Completeness [%]	93.4	90.4
Redundancy	4.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2		pH 6.2