4B34
Humanised monomeric RadA in complex with 2-amino benzothiazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.620, 78.770, 39.630 |
| Unit cell angles | 90.00, 118.06, 90.00 |
Refinement procedure
| Resolution | 33.220 - 1.550 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PZN CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.620 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.580 |
| Number of reflections | 29702 | |
| <I/σ(I)> | 16.5 | 2.2 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 50 MM NAKPHOSPHATE, PH 6.2 |
