4B30
Structure of the mutant V44A of the fluorescent protein KillerRed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 62 |
| Unit cell lengths | 134.310, 134.310, 76.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 116.320 - 2.100 |
| R-factor | 0.16715 |
| Rwork | 0.165 |
| R-free | 0.19858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wiq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.785 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 45765 | |
| <I/σ(I)> | 29.2 | 4.5 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 11 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP VAPOR DIFFUSION, A44-KR (15 MG/ML), 1.8 M MGSO4, 100 MM BICINE PH 8, 2% GLYCEROL |
