4B2P
RadA C-terminal ATPase domain from Pyrococcus furiosus bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.320, 62.020, 87.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.590 - 1.600 |
| R-factor | 0.18068 |
| Rwork | 0.179 |
| R-free | 0.21976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PZN CHAIN A |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.153 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.590 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 0.820 |
| Number of reflections | 29390 | |
| <I/σ(I)> | 17.79 | 3.06 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | pH 6.2 |
