4B2G
Crystal Structure of an Indole-3-Acetic Acid Amido Synthase from Vitis vinifera Involved in Auxin Homeostasis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.167, 91.167, 338.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.22646 |
Rwork | 0.224 |
R-free | 0.26387 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 338.070 | 2.500 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.130 | 0.790 |
Number of reflections | 59192 | |
<I/σ(I)> | 15.4 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.1 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | THE PROTEIN WAS CONCENTRATED TO 6 MG/ML IN THE PRESENCE OF 10 MM INHIBITOR AND CRYSTALLIZED AT 20 DEGREES CELSIUS IN 0.7 TO 1.0 M SODIUM MALONATE. |