4B29
Crystal structures of DMSP lyases RdDddP and RnDddQII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 145.540, 145.540, 145.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.910 - 1.720 |
| R-factor | 0.16247 |
| Rwork | 0.162 |
| R-free | 0.17572 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.746 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.810 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 54495 | |
| <I/σ(I)> | 22.3 | 6.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 1.2-1.4 M SODIUM/POTASSIUM PHOSPHATE AT PH 6.9 |
