4B27
Trp RNA-binding attenuation protein: modifying symmetry and stability of a circular oligomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.467, 110.151, 46.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.720 |
| R-factor | 0.20931 |
| Rwork | 0.207 |
| R-free | 0.24508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zzq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 14253 | |
| <I/σ(I)> | 15.3 | 2.8 |
| Completeness [%] | 91.0 | 68 |
| Redundancy | 6.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM BIS-TRIS PROPANE (PH 8.5), 200 MM KSCN AND 13% PEG 3350 (V/V) |
