4B20
Structural basis of DNA loop recognition by Endonuclease V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 55.231, 135.370, 194.993 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 2.750 |
R-factor | 0.2274 |
Rwork | 0.227 |
R-free | 0.29060 |
Structure solution method | OTHER |
RMSD bond length | 0.007 |
RMSD bond angle | 1.349 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 19328 | |
<I/σ(I)> | 10.6 | 2.1 |
Completeness [%] | 99.1 | 95.6 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |