4B1Y
Structure of the Phactr1 RPEL-3 bound to G-actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.580, 63.560, 62.570 |
| Unit cell angles | 90.00, 108.83, 90.00 |
Refinement procedure
| Resolution | 29.419 - 1.290 |
| R-factor | 0.1515 |
| Rwork | 0.150 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v52 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.296 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.360 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 98023 | |
| <I/σ(I)> | 5.5 | 1.8 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
