4B1W

Structure of the Phactr1 RPEL-2 domain bound to actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	I 4 2 2
Unit cell lengths	128.220, 128.220, 135.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.726 - 1.950
R-factor	0.1808
Rwork	0.179
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v52
RMSD bond length	0.007
RMSD bond angle	1.095
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.150	0.550
Number of reflections	38647
<I/σ(I)>	4	1.3
Completeness [%]	98.6	98.6
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1