4B1V
Structure of the Phactr1 RPEL-N domain bound to G-actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.121, 75.924, 122.451 |
| Unit cell angles | 90.00, 97.01, 90.00 |
Refinement procedure
| Resolution | 29.278 - 1.750 |
| R-factor | 0.1816 |
| Rwork | 0.179 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v52 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.165 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.010 | 0.530 |
| Number of reflections | 81005 | |
| <I/σ(I)> | 3.9 | 1.4 |
| Completeness [%] | 96.0 | 93.6 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
