4B1I
Structure of human PARG catalytic domain in complex with OA-ADP-HPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.687, 90.407, 94.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.260 - 2.140 |
| R-factor | 0.16235 |
| Rwork | 0.160 |
| R-free | 0.20713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.290 | 2.260 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 31210 | |
| <I/σ(I)> | 13.7 | 2.9 |
| Completeness [%] | 97.5 | 84.4 |
| Redundancy | 6.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 3350, 36.4MM PCTP-4, 63.6MM PCTP-10, 0.2M AMMONIUM SULPHATE |
