4B1H
Structure of human PARG catalytic domain in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.929, 90.656, 94.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.350 - 2.000 |
| R-factor | 0.20548 |
| Rwork | 0.204 |
| R-free | 0.24139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0d |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.414 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.540 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 39298 | |
| <I/σ(I)> | 10.8 | 2.5 |
| Completeness [%] | 98.9 | 90.8 |
| Redundancy | 6.15 | 3.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 3350, 36.4MM PCTP-4, 63.6MM PCTP-10, 0.2M AMMONIUM SULPHATE |
