4B10
Plasmodium vivax N-myristoyltransferase with a non-hydrolysable co- factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.370, 119.060, 176.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.830 - 1.560 |
| R-factor | 0.19062 |
| Rwork | 0.188 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wuu |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.123 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 161442 | |
| <I/σ(I)> | 15.2 | 2.2 |
| Completeness [%] | 98.0 | 82.8 |
| Redundancy | 5.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M AMMONIUM SULPHATE, 25% PEG 3350, 1 M BIS-TRIS PH 6.5 |
