4B0Z
Crystal structure of S. pombe Rpn12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.780, 91.380, 143.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.111 - 1.585 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.22480 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.525 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.340 | 1.670 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.050 | 0.500 |
| Number of reflections | 74706 | |
| <I/σ(I)> | 17.1 | 2.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M NA NO3, 0.1 M BIS-TRIS PROPANE PH 7.5, 22.5% PEG3350 |
