4B0Y
Determination of X-ray Structure of human SOUL by Molecular Replacement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 144.670, 144.670, 60.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.350 - 3.500 |
| R-factor | 0.2739 |
| Rwork | 0.270 |
| R-free | 0.30638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ayz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.846 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.350 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.150 | 0.750 |
| Number of reflections | 5025 | |
| <I/σ(I)> | 24.6 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 30.8 | 32.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2 M AMMONIUM SULPHATE, 0.1 M MES 6.5 |
