4B0F
Heptameric core complex structure of C4b-binding (C4BP) protein from human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.850, 73.850, 204.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.496 - 2.800 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24770 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 26577 | |
| <I/σ(I)> | 29.6 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.5 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 46.5 % (V/V) PEG 400, 5 % (V/V) MPD, 0.1 M TRIS PH. 7.3 WITH 13.7 MG/ML C4BP |
