4B0E
Crystal structure of the Caf1A usher protein N-terminal domain from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-02 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.858, 68.587, 192.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20487 |
| Rwork | 0.205 |
| R-free | 0.26135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INTERNAL STRUCTURE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.594 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.320 |
| Number of reflections | 27484 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 97.0 | 87 |
| Redundancy | 4.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | SITTING DROPS 1 MKL OF CAF1AN 6 MG/ML PLUS 1 MKL OF 20% PEG 3350, 0.2 M AMMONIUM NITRATE, PH 6.3 AT 20 DEG C, 2-6 MONTHS |
