4B02
The C-terminal Priming Domain is Strongly Associated with the Main Body of Bacteriophage phi6 RNA-Dependent RNA Polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 32 |
Unit cell lengths | 105.070, 105.070, 157.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.390 - 3.300 |
R-factor | 0.2198 |
Rwork | 0.218 |
R-free | 0.24190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hhs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.240 | 0.710 |
Number of reflections | 28332 | |
<I/σ(I)> | 6.7 | 1.6 |
Completeness [%] | 96.5 | 87 |
Redundancy | 4.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |