4B02
The C-terminal Priming Domain is Strongly Associated with the Main Body of Bacteriophage phi6 RNA-Dependent RNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 105.070, 105.070, 157.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.390 - 3.300 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.240 | 0.710 |
| Number of reflections | 28332 | |
| <I/σ(I)> | 6.7 | 1.6 |
| Completeness [%] | 96.5 | 87 |
| Redundancy | 4.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
