4AZL
In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.090, 245.760, 47.130 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 44.889 - 2.800 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rbh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.218 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.220 | 0.910 |
| Number of reflections | 23738 | |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 93.2 | 95.3 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM CACODYLATE, PH 6.5, 1.2 M SODIUM ACETATE |
