4AZ8
Crystal structure of the complex of the Caf1M:Caf1 chaperone:subunit preassembly complex carrying the KDKDTN insertion at the F1G1 loop region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-10 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.510, 69.308, 66.035 |
Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
Resolution | 29.722 - 2.650 |
R-factor | 0.2227 |
Rwork | 0.221 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p5v |
RMSD bond length | 0.006 |
RMSD bond angle | 1.441 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.110 | 0.370 |
Number of reflections | 9383 | |
<I/σ(I)> | 6.3 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, TRIS-HCL, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |