4AZ6
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.300, 109.600, 112.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.560 - 1.360 |
| R-factor | 0.1164 |
| Rwork | 0.115 |
| R-free | 0.13999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.810 | 1.430 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 93267 | |
| <I/σ(I)> | 19.1 | 4 |
| Completeness [%] | 90.1 | 57.7 |
| Redundancy | 9.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
