4AYS
The Structure of Amylosucrase from D. radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.180, 129.180, 122.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.619 - 3.150 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ucq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.743 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 18489 | |
| <I/σ(I)> | 7.7 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
