4AYP
Structure of The GH47 processing alpha-1,2-mannosidase from Caulobacter strain K31 in complex with thiomannobioside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 |
Unit cell lengths | 144.135, 144.135, 50.301 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.160 - 0.850 |
R-factor | 0.09692 |
Rwork | 0.096 |
R-free | 0.10555 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PREVIOUSLY SOLVED NATIVE STRUCTURE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.650 | 0.900 |
High resolution limit [Å] | 0.850 | 0.850 |
Rmerge | 0.040 | 0.570 |
Number of reflections | 333281 | |
<I/σ(I)> | 16.9 | 1.7 |
Completeness [%] | 97.2 | 80.8 |
Redundancy | 5.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M AMMONIUM ACTETATE, 0.1 M BIS-TRIS PH 6.5, 22% WT/VOL PEG 3350 |