4AY8
SeMet-derivative of a methyltransferase from M. mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 125.210, 125.210, 38.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.030 - 2.100 |
| R-factor | 0.14755 |
| Rwork | 0.146 |
| R-free | 0.17659 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 39734 | |
| <I/σ(I)> | 30.8 | 10.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 65% MPD |
