4AY0
High resolution crystal structure of the monomeric subunit-free Caf1M chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 56.767, 56.767, 248.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.765 - 1.520 |
| R-factor | 0.1668 |
| Rwork | 0.166 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A FROM PDB ENTRY 2OS7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.126 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.070 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 79538 | |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 8.52 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 40% PEG 600 AND 0.1 M CITRATE, PH 5.5 |
