4AXY
A molecular basis for the action of the collagen-specific chaperone Hsp47-SERPINH1 and its structure-specific client recognition.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.670, 45.670, 34.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.294 - 1.240 |
| R-factor | 0.1511 |
| Rwork | 0.150 |
| R-free | 0.17280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1051)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.310 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 10802 | |
| <I/σ(I)> | 20.6 | 3.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.75 | 3.15 M AMMONIUM SULFATE, 0.05 M CITRIC ACID PH 5.75 |
