4AXQ
CRYSTAL STRUCTURE OF ARCHAEMETZINCIN (AMZA) FROM ARCHAEOGLOBUS FULGIDUS AT 1.4 A RESOLUTION
Replaces: 2XHQExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 4 |
Unit cell lengths | 88.339, 88.339, 50.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.409 - 1.400 |
R-factor | 0.1652 |
Rwork | 0.164 |
R-free | 0.18640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x7m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.400 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.060 | 0.800 |
Number of reflections | 38202 | |
<I/σ(I)> | 15.77 | 1.99 |
Completeness [%] | 99.5 | 98.6 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 25% PEG 3350, 0.2 M (NH4)2SO4, 0.1 M HEPES PH 7.5 |