4AXI
Structure of the Clostridium difficile EutS protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2009-11-22 |
Detector | ADSC CCD |
Spacegroup name | H 3 |
Unit cell lengths | 123.200, 123.200, 38.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.930 - 1.510 |
R-factor | 0.1417 |
Rwork | 0.140 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cgi |
RMSD bond length | 0.016 |
RMSD bond angle | 1.679 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.600 | 1.590 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmerge | 0.060 | 0.500 |
Number of reflections | 34286 | |
<I/σ(I)> | 12.4 | 2.5 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | PROTEIN IN 200 MM NACL, 50 MM TRIS HCL PH 8.0 HANGING DROP VAPOUR DIFFUSION WITH 100/100 NL PROTEIN: PRECIPITANT OVER 100 UL WELL SOLUTION OF 24% (W/V) PEG 1500, 20% (W/V) GLYCEROL |