4AWH
Influenza strain pH1N1 2009 polymerase subunit PA endonuclease in complex with rUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.940, 120.110, 128.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.700 - 2.050 |
| R-factor | 0.20906 |
| Rwork | 0.207 |
| R-free | 0.24321 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w69 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.060 | 0.610 |
| Number of reflections | 53926 | |
| <I/σ(I)> | 15.71 | 2.29 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25 % PEG 3350, 0.1 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS PH5.5 |
