4AWD
Crystal structure of the beta-porphyranase BpGH16B (BACPLE_01689) from the human gut bacterium Bacteroides plebeius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 51.120, 107.230, 137.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.20261 |
Rwork | 0.200 |
R-free | 0.25014 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3juu |
RMSD bond length | 0.010 |
RMSD bond angle | 1.294 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.740 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.130 | 0.440 |
Number of reflections | 30374 | |
<I/σ(I)> | 10.5 | 4.2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 23-25 % PEG 3350, 100 MM TRIS PH 8.5 AND 5 MM CALCIUM CHLORIDE |