4AW7

BpGH86A: A beta-porphyranase of glycoside hydrolase family 86 from the human gut bacterium Bacteroides plebeius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	71.330, 87.700, 114.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.610 - 1.330
R-factor	0.13813
Rwork	0.137
R-free	0.15157
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.632
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.410
High resolution limit [Å]	1.330
Rmerge	0.060
Number of reflections	163194
<I/σ(I)>	15.8
Completeness [%]	99.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				27% PEG MME 2000, 0.1 M POTASSIUM THIOCYANATE AND 150 MM SODIUM IODIDE