4AVQ
Influenza strain pH1N1 2009 polymerase subunit PA endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 263.630, 66.240, 66.320 |
| Unit cell angles | 90.00, 95.98, 90.00 |
Refinement procedure
| Resolution | 49.130 - 2.100 |
| R-factor | 0.2365 |
| Rwork | 0.235 |
| R-free | 0.28386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w69 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 65996 | |
| <I/σ(I)> | 10.63 | 2.2 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.59 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.6 M SODIUM FORMATE 0.1 M HEPES PH 7 5% GLYCEROL |
