4AVF
Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.590, 116.590, 259.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.330 - 2.230 |
| R-factor | 0.18045 |
| Rwork | 0.179 |
| R-free | 0.20003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vrd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.242 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.000 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.080 | 0.750 |
| Number of reflections | 87734 | |
| <I/σ(I)> | 18.5 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 11% PEG 4000, 0.1M MES PH 6.5 |
