4AVD
C.lacteus nerve Hb in complex with CO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.660, 43.480, 60.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.730 - 1.500 |
| R-factor | 0.14713 |
| Rwork | 0.145 |
| R-free | 0.17876 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kr7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.320 |
| Number of reflections | 18465 | |
| <I/σ(I)> | 13.4 | 5.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 277 | 2.6 M AMMONIUM SULFATE, 50 MM SODIUM ACETATE PH 5.5, AT 277 K |
