4AVC
Crystal structure of protein lysine acetyltransferase Rv0998 in complex with acetyl CoA and cAMP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.661, 50.151, 110.102 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 39.884 - 2.806 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26720 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 17628 | |
| <I/σ(I)> | 11 | 1.91 |
| Completeness [%] | 98.5 | 97.5 |
| Redundancy | 2.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
