4AUV

Crystal Structure of the BRMS1 N-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-28
Detector	ADSC QUANTUM 210
Spacegroup name	C 2 2 21
Unit cell lengths	42.636, 191.274, 71.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.637 - 1.999
R-factor	0.196
Rwork	0.193
R-free	0.25850
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.019
RMSD bond angle	2.061
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.560	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.200
Number of reflections	20344
<I/σ(I)>	6.66	1.11
Completeness [%]	99.2	94.7
Redundancy	7.18	3.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.3		0.1 M SODIUM ACETATE PH 4.3, 0.2 M AMMONIUM SULPHATE, 2 % PEG 4000
1		4.3		0.1 M SODIUM ACETATE PH 4.3, 0.2 M AMMONIUM SULPHATE, 2 % PEG 4000