4AUA
Liganded X-ray crystal structure of cyclin dependent kinase 6 (CDK6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 |
| Unit cell lengths | 100.734, 100.734, 60.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.230 - 2.310 |
| R-factor | 0.21033 |
| Rwork | 0.207 |
| R-free | 0.27734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.354 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019G) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.230 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.370 |
| Number of reflections | 13277 | |
| <I/σ(I)> | 17.4 | 3.9 |
| Completeness [%] | 98.0 | 98.6 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 287 | 5MG/ML 0.1 M MES/NAOH PH 5.7,, 4.5% W/V PEG 3350, 25 MM SODIUM NITRATE, 10% V/V GLYCEROL, 10 DEGREES CELSIUS |
