4AU1
Crystal Structure of CobH (precorrin-8x methyl mutase) complexed with C5 desmethyl-HBA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-02 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.550, 65.710, 48.050 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 47.790 - 1.450 |
| R-factor | 0.1613 |
| Rwork | 0.161 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.790 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.030 | 0.460 |
| Number of reflections | 37503 | |
| <I/σ(I)> | 20.2 | 2.2 |
| Completeness [%] | 97.8 | 98.3 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
