4ATS

Structure of the ORF273 protein from the Acidianus two-tailed virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Collection date	2008-07-29
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 21 2
Unit cell lengths	101.013, 101.013, 52.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.920 - 3.850
R-factor	0.2719
Rwork	0.268
R-free	0.29260
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.010	3.930
High resolution limit [Å]	3.850	3.850
Rmerge	0.140	0.610
Number of reflections	2841
<I/σ(I)>	20.57	5.51
Completeness [%]	99.4	99.5
Redundancy	11.02	11.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		PROTEIN AT 5.5 MG/ML IN 20 MM HEPES (PH 7.5) AND 100 MM NACL WAS MIXED TO 5%-15% PEG 8000, 0.2 M MGCL2, 0.1 M TRIS(PH 7-8). CRYSTALS WERE OBTAINED BY THE HANGING-DROP VAPOUR-DIFFUSION METHOD