4ATO
New insights into the mechanism of bacterial Type III toxin-antitoxin systems: selective toxin inhibition by a non-coding RNA pseudoknot
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-28 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 6 |
Unit cell lengths | 127.100, 127.100, 37.735 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.950 - 2.200 |
R-factor | 0.1618 |
Rwork | 0.160 |
R-free | 0.19170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xdb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.083 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.230 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.050 | 0.550 |
Number of reflections | 34618 | |
<I/σ(I)> | 13.6 | 4.6 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 11 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | PRECIPITANT CONTAINS 0.2 M AMMONIUM PHOSPHATE, 0.1 M TRIS-HCL PH 8.5, 50% MPD. CRYSTALLIZATION WITH A SITTING DROP, VAPOUR DIFFUSION METHOD. |