4ATK

MITF:E-box complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	151.420, 45.090, 110.660
Unit cell angles	90.00, 136.47, 90.00

Refinement procedure

Resolution	26.072 - 2.950
R-factor	0.2551
Rwork	0.253
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ath
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	3.110
High resolution limit [Å]	2.950	2.950
Rmerge	0.070	0.560
Number of reflections	10446
<I/σ(I)>	11.8	2.3
Completeness [%]	94.7	94.4
Redundancy	4.7	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1